Klinisk prövning på Periodontitis: subgingival plaque sample, CGF

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Paul Savelkoul. Edwin Winkel. A. Van Winkelhoff. K. Boutaga. Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, 2018-09-19 In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s.

In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method. Curette sampling and a subgingival washing technique were compared within and between paired sites on two separate occasions in 24 patients using darkfield microscopy. Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non-motile organisms were obtained with subgingival washing compared with curette sampling. Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, clinical examinations were obtained prior to commencement of therapy for aggressive or generalized severe chronic periodontitis (n = 113), after combined mechanical and antibiotic anti‐infective subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes Bernadette Pretzl, Jule Paul, Diana M. Krigar, Lorenz Uhlmann, Peter Eickholz & Bettina Dannewitz Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?

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1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease.

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Supragingival plaque was removed prior to sampling using sterile cotton pellets.

78. Kageyama S Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with av N Schwech — Plaque samples were taken before pellicle, dental plaque, fluorometer, protease, gingivalvätska ger mineraler för mineralisering av subgingival tandsten. 57946T · Prevotella fusca · Human oral cavity, periodontic subgingival plaque · J.Downes, Kings College, London, UK · 2009-04-06 51937T · Prevotella multiformis · Human subgingival plaque · T.Kudo, JCM, Saitama, Japan · 2005-10-31 · 51939 · Streptomyces · Industry, environment Samples of subgingival plaque and gingival crevicular fluid were also taken. Plaque samples were analyzed with a DNA-DNA hybridization technique. T. forsythia, P. nigrescens and C. rectus in the sub-gingival and atherosclerotic plaque samples suggesting that the micoorganisms in the subgingival plaque The objective of this study was to determine whether the supragingival plaque samples of severe-ECC (S-ECC) patients harbor more EBV load than the plaque samples suggesting that the micoorganisms in the subgingival plaque had significant chance of their presence in atherosclerotic plaque as well Microbial analysis of subgingival plaque samples compared to that of Haririan, Hady; Andrukhov, Oleh; Bertl, Kristina; Lettner, Stefan; Kierstein, Sonja; Moritz, Sugar-free chewing gum helps neutralise plaque acids.
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Abstract. Background: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva 22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens. Blood and pus at the gingivalis in saliva and in subgingival plaque samples, showing comparable specificity to culture and real-time PCR. Conclusion: We applied the FRET technology 2 Jan 2018 DNA was then extracted from plaque samples and analyzed by 16S.

Author information: (1)Department of Periodontology, Center for Dentistry and Oral Medicine (Carolinum), Goethe University, Frankfurt, Germany. A Study to Evaluate Sampling Methods for Subgingival Plaque. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research.
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Significantly lower mean proportions of spirochetes and motile rods and higher proportions of non‐motile organisms were obtained with subgingival washing compared with curette sampling. The presence of specific bacteria in subgingival plaque has been used as an indicator of active periodontal disease. The technique of subgingival sampling may conjecturally influence the identification and enumeration of microorganisms reported. In this study, paper point sampling and scaler sampling are compared. Have minimum 6 sampling sites with bleeding and pocket depth ≥2mm but not deeper than 4mm.

Oral Microbiol Immunol 2008 23: 196-205. 16. Armitage GC. Development of a 3 Apr 2017 Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a broad obic culture and real-time PCR techniques. Methods: Pooled subgingival plaque samples and 10-ml mouthwash samples were collected from. 21 adult patients 31 Dec 2017 We thus focus on the use of paper points for subgingival sampling.
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Klinisk prövning på Subgingival Plaque: stannous fluoride

Bacterial diversity in human subgingival plaque.